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. 2013 Jul 23;2:e00868. doi: 10.7554/eLife.00868

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Copyright © 2013, Ducret et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (A) TEM images of lipid tubes deposited in the wake of a moving cell (left panel). A higher magnification view of lipid tubes/vesicles is shown in the right panel. Scale bars = 250 nm. (B) Deposition of lipid tubes/vesicles observed by an OM_sfGFP⁺/IM_mCherry⁺ cell. The deposited material is only stained with green fluorescence implying that it is derived from the OM. Scale bar = 1 µm. (C) Co-localization of deposited OM materials detected using OM_mCherry probe and slime detected using ConA-FITC. Corresponding phase contrast, red fluorescence, green fluorescence and overlay images are shown. Scale bar = 1 µm. (D) Lipid tubes/vesicles are embedded in the slime polymer (Black Arrow). Electron dense trails are clearly visible after ConA treatment. White arrows highlight gold particles specifically associated with biotinylated ConA and thus slime. Scale bar = 250 nm.

DOI: http://dx.doi.org/10.7554/eLife.00868.018

Figure 5—figure supplement 1. — (A) TEM images of lipid tubes deposited in the wake of a moving cell (left panel). A higher magnification view of lipid tubes/vesicles is shown in the right panel. Scale bars = 250 nm. (B) Deposition of lipid tubes/vesicles observed by an OM_sfGFP⁺/IM_mCherry⁺ cell. The deposited material is only stained with green fluorescence implying that it is derived from the OM. Scale bar = 1 µm. (C) Co-localization of deposited OM materials detected using OM_mCherry probe and slime detected using ConA-FITC. Corresponding phase contrast, red fluorescence, green fluorescence and overlay images are shown. Scale bar = 1 µm. (D) Lipid tubes/vesicles are embedded in the slime polymer (Black Arrow). Electron dense trails are clearly visible after ConA treatment. White arrows highlight gold particles specifically associated with biotinylated ConA and thus slime. Scale bar = 250 nm.

DOI: http://dx.doi.org/10.7554/eLife.00868.018