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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Hum Immunol. 2013 Apr 18;74(7):842–848. doi: 10.1016/j.humimm.2013.04.002

Fig. 4.

Fig. 4

Porcine CTLA-4-L97M mutant partially lost the binding ability to porcine CD80. (A) Flow cytometry binding analysis of porcine CTLA-4 (left panel) and porcine CTLA-4-L97M mutant (right panel) to the porcine CD80-expressing B-cell lymphoma line LCL 13271. PE-SA only; biotinylated protein control (porcine CD3εγ) [10]; isotype control (biotinylated Armenian hamster IgG, clone # HTK888, Biolegend) were included. (B) Bar graph presentation of the MFI from flow cytometry binding analysis data as described in (A). Different concentrations of biotinylated pCD3εγ were used as non-specific fluorescence control. Error bars are included based on the calculated standard deviation. The pCTLA-4-L97M mutant's ability to bind to porcine CD80 was significantly decreased compared to the pCTLA-4-WT, p < 0.05. (C) Porcine CD80 blocking analysis by flow cytometry of porcine CTLA-4 (left panel) and porcine CTLA-4-L97M mutant (right panel) with anti-CD80 mAb (clone 16-10A1) to the porcine CD80-expressing B-cell lymphoma line LCL 13271. The concentration of the competitor is indicated. All figures in (A)–(C) are representatives of multiple individual experiments.