Table 1.
Lys3 NH3+ | Lys55 NH3+ | Lys57 NH3+ | |
---|---|---|---|
Complex I a) | |||
- 800 MHz - | |||
15N R1 (s−1) | 0.25 ± 0.01 | 0.43 ± 0.02 | 0.74 ± 0.01 |
15N R2,ini (s−1) c) | 0.98 ± 0.05 | 1.98 ± 0.21 | 2.27 ± 0.04 |
1H-15N NOE | −2.40 ± 0.03 | −2.72 ± 0.08 | −3.00 ± 0.03 |
- 600 MHz - | |||
15N R1 (s−1) | 0.27 ± 0.01 | 0.59 ± 0.04 | 0.80 ± 0.02 |
1H-15N NOE | −3.07 ± 0.06 | −2.94 ± 0.17 | −3.17 ± 0.04 |
- Dynamics -d) | |||
S2axis | 0.22 ± 0.02 | 0.46 ± 0.06 | 0.48 ± 0.01 |
τf (ps) | 3 ± 1 | 23 ± 2 | 113 ± 42 |
τi (ps) | 222 ± 16 | 249 ± 42 | 82 ± 132 |
Complex II b) | |||
- 800 MHz - | |||
15N R1 (s−1) | 0.29 ± 0.02 | 0.43 ± 0.03 | 0.33 ± 0.02 |
15N R2,ini (s−1) c) | 1.16 ± 0.07 | 2.18 ± 0.26 | 1.76 ± 0.05 |
1H-15N NOE | −2.63 ± 0.06 | −2.72 ± 0.09 | −3.02 ± 0.13 |
- 600 MHz - | |||
15N R1 (s−1) | 0.29 ± 0.02 | 0.54 ± 0.08 | 0.36 ± 0.02 |
1H-15N NOE | −3.24 ± 0.10 | −3.08 ± 0.20 | −3.08 ± 0.13 |
- Dynamics -d) | |||
S2axis | 0.26 ± 0.02 | 0.49 ± 0.06 | 0.39 ± 0.01 |
τf (ps) | 8 ± 4 | 26 ± 12 | 27 ± 7 |
τi (ps) | 163 ± 43 | 184 ± 87 | 36 ± 19 |
With Duplex I (see Figure 1).
With Duplex II that contains a phosphorodithioate group at the ion pair with Lys57.
The initial rate for intrinsically bi-exponential 15N transverse relaxation13 of NH3+.
Symbols are defined in Figure 2a. The molecular rotational correlation time and anisotropy were determined to be 10.6 ns and 2.1, respectively, from backbone 15N relaxation data.