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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Dev Dyn. 2013 Feb 8;242(5):414–431. doi: 10.1002/dvdy.23926

Fig. 4. Migration defects upon loss of Hh pathway members in border cells.

Fig. 4

(A) Quantification of border cell migration at stage 10 upon knockdown of Hh pathway components using RNAi or overexpression approaches. Migration is shown as the percentage of border cells that migrated 0-25% (yellow), 26-75% (gray), or ≥76% (blue) of the distance to the oocyte. N ≥ 193 egg chambers for each genotype; at least 3 experiments were performed. Error bars represent standard error of the mean (SEM). (B-E) Fixed stage 10 egg chambers stained for phalloidin to mark F-actin (magenta) at cell membranes and DAPI (blue) to mark nuclei. GFP (green) marks wild-type cells in (E) and Fas III (green) marks polar cell membranes in (B, C). Arrows point to border cells. (B-D) Examples of egg chambers in which RNAi knockdown of smo (B) or ci (C) or in which ptc overexpression (D) is driven by slbo-GAL4; all show incomplete border cell migration. (E) Loss of smo by mosaic clonal analysis reveals a migration defect in a stage 10 egg chamber. Inset shows that two cells in the border cell cluster are mutant (GFP-negative; arrowheads). Scale bar in (B) represents 20 μm.