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. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: Am J Transplant. 2012 Jan 10;12(4):867–876. doi: 10.1111/j.1600-6143.2011.03917.x

Figure 4. B cell deficiency results in a significant reduction in the frequency of IFN-γ and IL-17 secreting T cells against K-α1T and ColV in the lung following anti-MHC Ab administration.

Figure 4

B6.129S2-Igh-6tm1Cgn/J (B cell KO) or control C57BL/6 mice were treated with anti-MHC class I Abs on day 1, 2, 3, 6 and weekly thereafter. T cells infiltrating the lungs were harvested by collagenase digestion and the frequency of T cells secreting IFN-γ, IL-4 and IL-17 on stimulation with K-α1T and ColV (3×105 cells/well cultured in triplicate for each antigen) were analyzed by ELISPOT. A significant reduction in the frequency of T cells against K-α1T and ColV was observed in B cell deficient mice when compared to controls. IFN-g secreting cells to LPS in vitro did not differ among the groups. Data represented as mean ± SD spots per million cells (spm) of 5 mice per group.