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. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: Am J Transplant. 2012 Jan 10;12(4):867–876. doi: 10.1111/j.1600-6143.2011.03917.x

Figure 6.

Figure 6

A: Decreased auto-Ab production in B cell deficient mice following anti-MHC Ab administration. B6.129S2-Igh-6tm1Cgn/J (B cell KO) or control C57BL/6 (wild type) mice were treated with anti-MHC class I Abs day1, 2, 3, 6 and weekly thereafter. Serum collected at day 30 was analyzed for auto-Abs against K-α1T and ColV by ELISA. Abs to K-α1T and ColV were significantly reduced in B cell deficient mice when compared to wild type following administration of anti-MHC Abs. Represented as mean ± SD μg/mL of 5 mice per group. * Denotes p<0.05

B: Formation of germinal centers is significantly reduced in the B cell deficient mice following administration of anti-MHC Abs when compared to wild type controls. B6.129S2-Igh-6tm1Cgn/J (B cell KO) or control C57BL/6 (wild type) mice were treated with anti-MHC class I Abs day 1, 2, 3, 6 and weekly thereafter. Spleens from the mice were harvested on day 30 and analyzed for the germinal center formation by staining with anti-PNA Abs. Immunohistochemical analysis of the spleen from the wild type mice demonstrated significantly increased number of germinal centers in comparison to B cell deficient mice.