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Four-color flow cytometry for the frequency of tetramer (tet)+CD3+CD8+ T cells in the peripheral circulation of a patient with HNC. The frequency of wt p53264-272 peptide-specific and viral peptide-specific T cells in the peripheral blood of a representative HLA-A*02+ patient with HNC is shown. The frequency values for tet+CD8+ T cells are indicated in the upper right quadrants.
