Skip to main content
. 2013 Jul 9;109(2):370–378. doi: 10.1038/bjc.2013.319

Table 2. Antibodies used for immunohistochemistry (IHC) and western blot (WB)/RPPA analysis.

Protein Antibody Distributor IHC Dilution WB/RPPA
HSP27
#2402
Cell Signaling, Danvers, MA, USA
1 : 250
1 : 1000
Phospho-HSP(Ser15)
#ab39399
Abcam, Cambridge, UK
1 : 500
1 : 1000
Phospho-HSP(Ser78)
#2405
Cell Signaling, Danvers, MA, USA
ND
1 : 1000
Phospho-HSP(Ser82)
#2401
Cell Signaling, Danvers, MA, USA
ND
1 : 1000
HSP60
#ab46798
Abcam, Cambridge, UK
1 : 2000
1 : 2000
HSP70
#ab17850
Abcam, Cambridge, UK
1 : 1
1 : 50
HSP90
#ab1429
Abcam, Cambridge, UK
1 : 100
1 : 200
GRP78
#ab32618
Abcam, Cambridge, UK
1 : 1000
1 : 1000
GRP94 #sc1794 Santa Cruz Biotechnology Inc., CA, USA 1 : 5000 1 : 500

Abbreviations: GRP=glucose-related protein; HSP=heat-shock protein; ND= not done; RPPA=reverse phase protein array.