Table 2. Antibodies used for immunohistochemistry (IHC) and western blot (WB)/RPPA analysis.
Protein | Antibody | Distributor | IHC Dilution | WB/RPPA |
---|---|---|---|---|
HSP27 |
#2402 |
Cell Signaling, Danvers, MA, USA |
1 : 250 |
1 : 1000 |
Phospho-HSP(Ser15) |
#ab39399 |
Abcam, Cambridge, UK |
1 : 500 |
1 : 1000 |
Phospho-HSP(Ser78) |
#2405 |
Cell Signaling, Danvers, MA, USA |
ND |
1 : 1000 |
Phospho-HSP(Ser82) |
#2401 |
Cell Signaling, Danvers, MA, USA |
ND |
1 : 1000 |
HSP60 |
#ab46798 |
Abcam, Cambridge, UK |
1 : 2000 |
1 : 2000 |
HSP70 |
#ab17850 |
Abcam, Cambridge, UK |
1 : 1 |
1 : 50 |
HSP90 |
#ab1429 |
Abcam, Cambridge, UK |
1 : 100 |
1 : 200 |
GRP78 |
#ab32618 |
Abcam, Cambridge, UK |
1 : 1000 |
1 : 1000 |
GRP94 | #sc1794 | Santa Cruz Biotechnology Inc., CA, USA | 1 : 5000 | 1 : 500 |
Abbreviations: GRP=glucose-related protein; HSP=heat-shock protein; ND= not done; RPPA=reverse phase protein array.