Figure 3.

The FAN-deficient B16 cells display reduced invasive activity in vivo. (A) Survival of wild-type mice after subcutaneous (s.c.) injection with B16 mouse melanoma cells. (B) Dissected lungs of mice injected with wt and FAN-deficient B16 melanoma cells (n=6). On day 16, the lungs were harvested and the number of macroscopically visible tumours on the surface of the lungs was counted independently by two investigators. (C) Zebrafish were injected in the yolk sac with either F10 wt cells or F10shFANC2 cells. Arrows indicate disseminated tumour cells. (D) Quantification of cell dissemination. The number of cells that disseminated into the body were counted independently by two investigators (n=5 fish per cell line). Results are expressed as mean±s.e.m. (E) Survival of zebrafish embryos after tumour cell injection. Zebrafish embryos injected with the FAN-deficient cell lines display a higher survival rate compared with embryos injected with wt cells 48 hpi (hours post injection).