Decynium22 (right panel; green cells) was accumulated by the same cells that had previously accumulated ASP+ (middle panel; red cells). From left to right: 40x oil immersion image of cultured astrocytes in bright field; accumulation of ASP+ fluorescence in the same astrocytes population (middle panel, red), accumulation of D22 fluorescence (right panel, green). Please note, that cells, that have not accumulated ASP+ also have no accumulation of decynuim22 (D22, and vise versa, cells that accumulate one of these dyes, also accumulated the other one). Note: that ASP+ and D22 probably share the same transporter pathway.