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. 2013 Jun 3;5(7):949–966. doi: 10.1002/emmm.201202318

Figure 2. MiR-146a and miR-146b expression is sustained after the removal of IL-1β.

Figure 2

Endothelial cells were treated with IL-1β for 24 h, after which IL-1β was removed. Levels of VCAM-1 and SELE (A), mature miR-146a/b (B) and pri-miR-146a/b (C) were monitored at various time-points after the removal of IL-1β by qRT-PCR. While VCAM-1 and SELE rapidly returned to base-line levels, miR-146a/b levels remained elevated. The transcription of miR-146a decreased by 24 h after removal of IL-1β, while transcription of miR-146b was sustained in the absence of IL-1β. Data represents the mean ± SEM of three independent experiments. Statistical analyses were performed using t-test to compare post-IL-1β removal time-points to 24 h of IL-1β treatment (i.e., time zero). Significant p-values (from left to right) in (A) are 0.0004, <0.0001, 0.0004, <0.0001, 0.0006 and 0.0001, respectively. p-value in (B) is 0.049. p-Values in (C) are 0.012, 0.001 and 0.001, respectively.