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. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: N Engl J Med. 2011 Aug 4;365(5):430–438. doi: 10.1056/NEJMoa1103975

Figure 3. Lymphodepletion after ATG administration.

Figure 3

(A) The initial decrease in total absolute lymphocyte count was similar between the two ATGs, but lymphocyte counts remained lower longer after rabbit ATG. (B) T cells (CD3+CD45+) decreased rapidly with both ATGs with reconstitution in subsequent weeks. (C, D) There was a large difference between the kinetics of CD4+ T cell depletion after horse and rabbit ATG, with a much lower frequency and absolute numbers after rabbit ATG. (E, F) The frequency of regulatory T cells (Tregs; defined as CD4+CD25+CD127− for this analysis) was higher after rabbit ATG, but absolute numbers were markedly lower due to more potent depletion of CD4+ T cells, as compared to horse ATG. (G, H) The difference in depletion kinetics of CD8+ T cells was less striking between the two ATGs when compared to CD4+ T cells. The mean ± standard error of mean is shown. All 120 patients are depicted in panel A. Fourteen patients are depicted in panels B–H (7 from each ATG group). Differences for each time point that are statistically significant (p<0.05) are denoted by an asterisk (paired t-test). For details on flow cytometric analysis, see Methods section in Supplementary Appendix.