Figure 4. Sin3A^lof flies are resistant to rotenone-induced locomotor impairment.

Wild type (CS) and Sin3A^lof flies on vehicle-supplemented control food had comparable negative geotactic response. Exposure to 125 μM rotenone for 3 days caused a significant decrease in negative geotactic response in wild type flies. However, Sin3A^lof flies reared on 125 μM rotenone for 3 days had comparable negative geotactic response to Sin3A^lof flies on vehicle-supplemented control food. Additionally, Sin3A^lof flies reared on 125 μM rotenone for 3 days had a significant increase in the odds of reaching the top of the apparatus as compared to wild type flies on the same treatment. Columns=average percentage of flies reaching the top section of the apparatus after 1 min; error bars=standard error; Odds ratio=odds ratio for mobility to top section as compared to without SB control for the same rotenone dose; * p<0.05, ** p<0.01, *** p<0.001 for odds of survival; n=3–8 biological replicates set in at least duplicates.