Figure 6. SB treatment reduces dopamine deficiency in rotenone-exposed flies.

(a) Wild type (CS) and Sin3A^lof flies on vehicle-supplemented control food had comparable levels of dopamine in the head extract. Exposure to 125 μM rotenone for 3 days caused a significant decrease in head dopamine levels as compared to vehicle only in both wild type and Sin3A^lof flies. However, rotenone-exposed Sin3A^lof flies had higher dopamine levels (not significant) as compared to rotenone-exposed wild type flies. In the presence of rotenone, 10 mM SB treatment for 3 days showed a significant improvement in head dopamine level in wild type flies only. n=3 biological replicates. (b) Serotonin levels were measured in the same groups of flies as in (a). No significant differences were observed in the serotonin content among the groups. Columns=average dopamine or serotonin content; error bars=standard error; n=3 biological replicates; * p<0.05, *** p<0.001; ANOVA; Scheffe post-hoc test; p=0.73 (serotonin).