Figure 5. Engraftment of GM-CSF knock-down Kras^G12D-PDEC is accompanied by an increase in infiltrating CD8⁺ T cells.

(A) Immunohistochemical staining and quantification of CD8 and cleaved caspase 3 staining on sections of scr- and GM-sh GFP-Kras^G12D-PDEC orthotopic grafts at 2 weeks post-implantation. CD8⁺ or cleaved caspase3-positive cells within the boundaries of orthotopic grafts were counted per field of view (FOV) at 20x magnification. White arrowheads indicate CD8⁺ cells; black arrowheads indicate caspase-3-positive cells. Scale bars, 50μm. Error bars indicate SD, (n = 4 mice per group, 4 FOV per mouse).

(B) Immunohistochemical staining for CD8 and B220 on consecutive sections of scr- and GM-sh GFP-Kras^G12D-PDEC orthotopic grafts at 4 weeks post-implantation. Numbers of CD8⁺ cells were counted per field of view (FOV) at 20x magnification and are shown in the graph. Arrows indicate co-aggregation of CD8⁺ cells and B220⁺ cells. Scale bar, 100μm. Error bars indicate SD, (n = 3 mice per group, 5 FOV per mouse). p value: ***<0.001. See also Figure S4.