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. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: Cancer Cell. 2012 Jun 12;21(6):836–847. doi: 10.1016/j.ccr.2012.04.024

Figure 6. CD8+ T cells are instrumental in the clearance of GM-CSF knock-down KrasG12D-PDEC.

Figure 6

(A) Orthotopic grafts formed by scr- or GM-sh GFP-KrasG12D-PDEC implanted into either mock-depleted (IgG) or CD8-depleted animals were analyzed at 2 weeks post-implantation by H&E. Black arrowheads indicate pancreatic ductal structures. Scale bar, 100μm.

(B) The extent of colonization of the grafted areas by scr- or GM-sh GFP-KrasG12D-PDEC in mock-depleted (IgG) or CD8-depleted animals at 2 weeks post-implantation was analyzed by immunohistochemistry for GFP. Scale bar, 100μm.

(C) Graph depicts quantification of the data from (B) and indicates the fraction of GFP+ area per total area of the graft. Error bars indicate SD, (n = 4 mice per group, 5 FOV per mouse)

p value: ***<0.001, NS–not significant. See also Figure S5.