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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Am Chem Soc. 2013 Apr 17;135(17):6529–6540. doi: 10.1021/ja4005943

Figure 1.

Figure 1

Cyanophenylalanine/Thioamide FRET Interactions. Top Left: Cyanophenylalanine (Cnf, F*) and leucyl thioamide (Leu′, L′). Top Right: Normalized absorption (dotted black line) and fluorescence emission (dashed black line) spectrum of Cnf and absorption spectrum of Leu′Ala (yellow line). The shaded area indicates the spectral overlap that contributes to FRET. Bottom: Distance (RFRET) calculations are made using Förster theory as described in text.