Figure 1.
Effect of restraint stress on HSV-1-induced vaginal pathology. Mice were infected intravaginally with 5 × 106 pfu HSV-1. Control mice were mock-infected with phosphate-buffered saline (PBS) (A, B). Food-and-water deprivation (C, D) or restraint stress (E, F) began one day prior to infection and continued daily thereafter. Cross-sections (7 μm) were observed at 10× (A, C, E) and 20× (B, D, F) using an Olympus IX81 deconvolution microscope and QCapture software. A representative field is presented for each condition.