Figure 1.
Effect of stress on CD4+ and CD8+ T lymphocyte populations within the NALT (A), MLN (B), and CLN (C). Mice were subjected to food-and-water deprivation (FWD; black bars) or restraint stress (STRESS; white bars) for six consecutive sessions. Immediately following the sixth session, mice were euthanized and lymphocytes from the NALT, MLN, and CLN were isolated and quantified as described in the Methods. The numbers of NALT-derived lymphocytes for restraint mice are expressed as a percentage of the FWD control mice, since the numbers of NALT-derived lymphocytes varied among experiments. n = 5–9 for all groups. * Significant difference (p < 0.05) as compared to FWD control mice.