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. 2013 Jul 24;33(30):12275–12286. doi: 10.1523/JNEUROSCI.5919-12.2013

Figure 8.

Figure 8.

Overexpression of CLHM-1 in the body-wall muscles causes lethality. A–D, Bright-field (A, B) and fluorescent images (C, D) of transgenic animals, identified by DsRed2 expression, with clhm-1 overexpressed in the body-wall muscles. A, C, Animals grown on empty-vector RNAi. B, D, Animals grown on clhm-1 RNAi. Arrowheads indicate nontransgenic animals that lack the DsRed2 marker; arrows indicate transgenic animals. Scale bars: 1000 μm. E, Number of viable transgenic lines isolated when clhm-1 was expressed from the body-wall muscle myo-3 promoter and the endogenous clhm-1 promoter. F, Growth of myo-3 promoter::clhm-1-overexpressing worms on the indicated RNAi bacterial clones. Normalized time of flight is a measure of worm length that corresponds to developmental stage. n ≥ 3 independent experiments per RNAi clone; n > 1000 animals per experiment. Data shown are mean ± SEM. **p < 0.01; ***p < 0.001.