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. 2013 Jul 24;33(30):12306–12315. doi: 10.1523/JNEUROSCI.5220-12.2013

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Copyright © 2013 the authors 0270-6474/13/3312306-10$15.00/0

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Figure 3. — CSAS protein is modified with N-linked glycans when expressed in vivo. Western-blot analysis of CSAS-FLAG expressed in Drosophila heads. Lane 1, Cell lysate from heads. Lane 2, Affinity purified CSAS-FLAG, PNGase F mock-treated control. Lane 3, Affinity purified CSAS-FLAG treated with PNGase F. The decrease of CSAS molecular mass upon PNGase treatment indicates the presence of N-linked glycans. Ig^HC and Ig^LC indicate Ig chains leached from FLAG-affinity beads in denaturing conditions. Approximate positions of molecular mass markers are shown on the left.