Figure 5.

CSAS mutations cause TS paralysis. TS-paralysis assays of flies with different combinations of CSAS alleles. CSAS^Mi mutants have a slightly milder paralysis phenotype than CSAS²¹, which suggests that CSAS^Mi is a strong hypomorphic mutation. At least 20 flies were assayed for each genotype. WT, Wild-type control; CSAS^Mi/Mi, CSAS^Mi homozygous mutant; CSAS^21/21, CSAS²¹ homozygous mutant; CSAS^Mi/21 and CSAS^Mi/Df, heteroallelic CSAS mutants carrying combinations of CSAS mutant alleles; CSAS^R3/R3, homozygous for a revertant allele; CSAS⁺ CSAS^Mi/Df, CSAS^Mi/Df mutant rescued by transgenic expression of CSAS-FLAG. Statistical analysis was performed using one-way ANOVA followed by post hoc multiple-comparison Wilcoxon each pair test. *p < 0.05. Error bars represent SEM.