Figure 6.

CSAS mutants have a defect of neural transmission at larval NMJ. A, Evoked EJP amplitudes recorded from abdominal muscle 1 in third instar larvae. EJP is significantly reduced in CSAS^Mi and CSAS²¹ mutants, compared with wild-type larvae. CSAS⁺ CSAS^Mi/Mi, EJP defect was rescued in CSAS^Mi homozygous mutants by transgenic expression of CSAS-FLAG. At least 14 larvae were analyzed for each genotype. Statistical analysis was performed by one-way ANOVA followed with multiple-comparison Wilcoxon each pair test. *p < 0.05, ***p < 0.001, statistically significant differences. B, Representative traces of evoked EJPs. C, The evoked EJP defect of CSAS mutants can be rescued by increased Ca²⁺ concentration. Ten larvae were assayed for each data point. Error bars represent SEM. See Figure 5 for explanation of genotypes.