Table 2.
Secondary antibodies used in this study
| Secondary antibody | Catalog No. | Isotype | Host | Anti- | Dilution for immunohistochemistry | Dilution for Western blot | Company |
|---|---|---|---|---|---|---|---|
| IRDye 800CW | 926-32210 | IgG (heavy and light chains) | Goat | Mouse | 1:2000 | Odyssey (LI-COR) | |
| IRDye 800CW | 926-32213 | IgG (heavy and light chains) | Donkey | Rabbit | 1:2000 | Odyssey (LI-COR) | |
| IRDye 680LT | 926-68020 | IgG (heavy and light chains) | Goat | Mouse | 1:2000 | Odyssey (LI-COR) | |
| IRDye 680LT | 926-68023 | IgG (heavy and light chains) | Donkey | Rabbit | 1:2000 | Odyssey (LI-COR) | |
| IRDye 680LT | 926-68031 | Conjugate streptavidin | Biotinylated antibody | 1:1000 | Odyssey (LI-COR) | ||
| Rhodamine | 111-295-144 | IgG (heavy and light chains) | Goat | Rabbit | 1:250 | Jackson ImmunoResearch Laboratories | |
| Cy5 | 111-605- Immuno144 | IgG (heavy and light chains) | Goat | Rabbit | 1:200 | Jackson ImmunoResearch Laboratories | |
| Alexa Fluor 647 | A31573 | IgG (heavy and light chains) | Donkey | Rabbit | 1:300 | Invitrogen | |
| Alexa Fluor 647 | A21237 | IgG (heavy and light chains) | Goat | Mouse | 1:300 | Invitrogen | |
| Alexa Fluor 546 | A10036 | IgG (heavy and light chains) | Donkey | Mouse | 1:500 | Invitrogen | |
| Alexa Fluor 546 | A11071 | IgG (heavy and light chains) | Goat | Rabbit | 1:500 | Invitrogen | |
| Alexa Fluor 594 | S32356 | Conjugate streptavidin | Biotinylated antibody | 1:500 | Invitrogen | ||
| Alexa Fluor 647 | S32357 | Conjugate streptavidin | Biotinylated antibody | 1:300 | Invitrogen |