Table 1. Proteins Identified In Obese Female Mice: Mitochondrial Energy Metabolism.
Spot | SSP | Mr (Da) | pI | Mascot score | Protein name | Swiss prot accession number | % Sequence coverage | No of peptides | Fold increase or decrease | Functional group or function |
1 | 2305 | 56265 | 5.19 | 873 | ATP synthase subunit beta, | P56480 | 44 | 18 | 2.7 | ATP synthesis, (catalytic subunit) |
2* | 5002 | 18664 | 5.52 | 273 | ATP synthase D chain | Q9DCX2 | 70 | 11 | 2.5 | ATP synthesis, (rotational mechanism) |
3* | 6003 | 53420 | 5.75 | 280 | Ubiquinol cytochrome-C reductase core protein 1 | Q9CZ13 | 21 | 7 | –2.1 | Electron transport |
4 | 8406 | 59830 | 9.22 | 693 | ATP synthase subunit alpha | P56757 | 30 | 15 | 14.9 | ATP synthesis (regulatory subunit) |
5 | 3101 | 27640 | 5.19 | 297 | NADH dehydrogenase flavoprotein 2 | Q9D6J6 | 45 | 9 | –1.8 | Electron transfer |
6 | 2903 | 80724 | 5.51 | 563 | NADH ubiquinone oxidoreductase 75 kDa subunit | Q91VD9 | 26 | 18 | –2 | Electron transfer |
7* | 9302 | 35360 | 8.62 | 623 | Electron transfer flavoprotein subunit alpha | Q99LC5 | 51 | 15 | 5.9 | Electron transfer |
Data represent differentially expressed proteins between control and obese female mice. Only proteins that were significantly different are reported (p < 0.05). The SSP number is assigned by the PDQuest software program (Bio-Rad, Hercules CA). *Proteins identified in two or more independent experiments.