Table 2. Proteins Identified In Obese Females: Contraction/Cytoskeletal.
Spot | SSP | Mr (Da) | pI | Mascot score | Protein name | Swiss prot accession number | % Sequence coverage | No of peptides | Fold increase or decrease | Functional group or function |
8 | 0203 | 32718 | 4.69 | 429 | Tropomyosin 1, alpha chain | P58771 | 34 | 11 | 2.5 | Striated muscle contraction |
9* | 3201 | 42381 | 5.23 | 268 | Actin, smooth muscle | P62737 | 22 | 7 | –2.3 | Structural constituent of cytoskeleton |
10* | 3203 | 42334 | 5.23 | 318 | Actin, alpha cardiac | P68033 | 35 | 10 | –4.0 | Structural constituent of cytoskeleton |
11* | 7002 | 224349 | 5.57 | 225 | Myosin heavy chain | QO2566 | 2 | 6 | –3.8 | Muscle contraction |
12* | 5401 | 141799 | 6.06 | 179 | Myosin binding protein C, cardiac | O70468 | 6 | 9 | –1.9 | Muscle contraction |
13 | 0206 | 22390 | 5.03 | 283 | Myosin light chain | P05977 | 33 | 8 | 1.7 | Muscle contraction |
Data represent differentially expressed proteins between control and obese female mice. Only proteins that were significantly different are reported (p < 0.05). The SSP number is assigned by the PDQuest software program (Bio-Rad). *Proteins identified in two or more independent experiments.