Table 1.

Identified BSA tryptic peptides from various MWCO separation conditions

BSA tryptic peptide (MH+)	100% H2O 1 μg	100% 1 M NaCl	70% H2O	80% 1 M NaCl	70% 1 M NaCl	60% H2O	60% 1 M NaCl
508.3	✓	✓	✓		✓	✓	✓
545.3			✓	✓	✓	✓	✓
689.4				✓	✓	✓	✓
712.4			✓	✓		✓	✓
898.5	✓	✓	✓	✓	✓	✓	✓
927.5			✓	✓	✓	✓	✓
1034.5							✓
1072.5				✓			✓
1138.5				✓			✓
1163.6					✓	✓	✓
1249.6
1283.7					✓		✓
1305.7			✓		✓	✓	✓
1399.7
1415.7
1419.7							✓
1439.8
1463.6				✓		✓	✓
1479.8					✓	✓	✓
1502.6						✓	✓
1511.8							✓
1532.8							✓
1554.7					✓		✓
1567.7
1576.8						✓	✓
1639.9					✓	✓	✓
1667.8							✓
1673.8
1724.8
1740.8
1747.7							✓
1749.7					✓	✓	✓
1880.9					✓	✓	✓
1889.0
1901.9					✓		✓
1907.9					✓
2045.0
2113.9
2247.9
Total: 39	2	2	6	8	15	15	27

The percentage listed represents the percent of aqueous present with the remaining being MeOH. The 100% H₂O MWCO separation used 1 μg of BSA tryptic peptide standard, while all the other listed conditions used 500 ng. The column titled BSA tryptic peptide refers to a directly spotted BSA tryptic digest and the masses matched to tryptic peptides. A checkmark indicates a tryptic peptide was mass matched and present at the indicated m/z with a ¹³C isotope peak. The numbers of BSA tryptic peptides identified are listed at the bottom of the table.