Table 1.
BSA tryptic peptide (MH+) | 100% H2O 1 μg |
100% 1 M NaCl |
70% H2O |
80% 1 M NaCl |
70% 1 M NaCl |
60% H2O |
60% 1 M NaCl |
---|---|---|---|---|---|---|---|
508.3 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |
545.3 | ✓ | ✓ | ✓ | ✓ | ✓ | ||
689.4 | ✓ | ✓ | ✓ | ✓ | |||
712.4 | ✓ | ✓ | ✓ | ✓ | |||
898.5 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
927.5 | ✓ | ✓ | ✓ | ✓ | ✓ | ||
1034.5 | ✓ | ||||||
1072.5 | ✓ | ✓ | |||||
1138.5 | ✓ | ✓ | |||||
1163.6 | ✓ | ✓ | ✓ | ||||
1249.6 | |||||||
1283.7 | ✓ | ✓ | |||||
1305.7 | ✓ | ✓ | ✓ | ✓ | |||
1399.7 | |||||||
1415.7 | |||||||
1419.7 | ✓ | ||||||
1439.8 | |||||||
1463.6 | ✓ | ✓ | ✓ | ||||
1479.8 | ✓ | ✓ | ✓ | ||||
1502.6 | ✓ | ✓ | |||||
1511.8 | ✓ | ||||||
1532.8 | ✓ | ||||||
1554.7 | ✓ | ✓ | |||||
1567.7 | |||||||
1576.8 | ✓ | ✓ | |||||
1639.9 | ✓ | ✓ | ✓ | ||||
1667.8 | ✓ | ||||||
1673.8 | |||||||
1724.8 | |||||||
1740.8 | |||||||
1747.7 | ✓ | ||||||
1749.7 | ✓ | ✓ | ✓ | ||||
1880.9 | ✓ | ✓ | ✓ | ||||
1889.0 | |||||||
1901.9 | ✓ | ✓ | |||||
1907.9 | ✓ | ||||||
2045.0 | |||||||
2113.9 | |||||||
2247.9 | |||||||
Total: 39 | 2 | 2 | 6 | 8 | 15 | 15 | 27 |
The percentage listed represents the percent of aqueous present with the remaining being MeOH. The 100% H2O MWCO separation used 1 μg of BSA tryptic peptide standard, while all the other listed conditions used 500 ng. The column titled BSA tryptic peptide refers to a directly spotted BSA tryptic digest and the masses matched to tryptic peptides. A checkmark indicates a tryptic peptide was mass matched and present at the indicated m/z with a 13C isotope peak. The numbers of BSA tryptic peptides identified are listed at the bottom of the table.