Skip to main content
. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: Nat Struct Mol Biol. 2011 Dec 11;19(1):113–116. doi: 10.1038/nsmb.2179

Figure 2.

Figure 2

TriPol replisomes are more efficient on the lagging strand than DiPol replisomes are. (a) Scheme of the bead-based assay; the DiPol replisome is illustrated for simplicity. (b) Dipol and Tripol replisomes replicate DNA with similar rates. Left, autoradiogram of 0.8% alkaline agarose gel analysis of reactions, using either Tripol III* (20 nM) or DiPol III* (80 nM); DnaG primase concentration was 200 nM. Right, plot of DNA length versus time. (c) Left, leading- and lagging-strand replication products from bead-based reactions, resolved on denaturing agarose gels, using 320 nM DnaG primase. Right, quantitation of leading- and lagging-strand synthesis, normalized to the products of the TriPol replisome.