Fig. 7.

(a) Line 66.1 tumor cells were treated with FA (1μM) or PBS control for 24h, washed and 1×10⁵ viable cells injected intravenously into Balb/cByJ mice or Balb/cByJ mice depleted of NK cells with asialoGM1 antibody. Twenty-one days later, mice were euthanized and surface lung tumor colonies were enumerated. Mean ± SE of lung metastases in 10 mice/group. (b) N-NK cells were pretreated with PGE₂, PGE₁-OH, and FA (0.1μM) with and without EP receptor agonists and cell culture supernatants were assayed for IFNγ. Results are reported as mean pg/mL/1×10⁶ cells ± standard error. (c) N-NK cells were pretreated with FA (0.1μM and 0.5μM) in the presence and absence of PGE₂ and PGE₁-OH and migration capacity was determine by the number of cells that crossed a 3μm pore membrane. Data reported as mean number of cells migrated ± standard error. Relative to DMSO, treatment *p<0.05.