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Cytotoxic effect of the supernatant of wild-type and isogenic mutants on bovine umbilical vein endothelial cells (BUVEC.) Photomicrograph of Haemacolor-stained cells after 1.5 h exposure to 3% supernatant of the wild-type strain (WT), ΔpfoA Δplc double mutant (ΔpfoA Δplc), pfoA-complemented ΔpfoA Δplc mutant (pfoA-c.), plc-complemented ΔpfoA Δplc mutant (plc-c.), double-complemented ΔpfoA Δplc mutant (double-c.) and SFM as a negative control. Magnification, ×400.
