Table 1.
Description of strains and toxin activities in supernatants of an overnight culture
| Straina | Strain number | Relevant characteristicsb | Plasmid-encoded toxin gene(s)a | Plc activity(μg mL-1)c | PFO activity (log2(titre))c | Ref. |
|---|---|---|---|---|---|---|
| Wild-type |
JIR325 |
Strain 13d |
|
12.5 ± 2.5 |
4.8 ± 0.2 |
[25] |
| ΔpfoAΔplc |
JIR4444 |
JIR325 pfoA::ermB plcΩpJIR1774, suicide plasmid |
|
< 1.0 |
< 1.0 |
[23] |
|
pfoA-complemented ΔpfoAΔplc |
JIR4460 |
JIR4444(pJIR871), CmR |
pfoA+ |
< 1.0 |
4.1 ± 0.3 |
[23] |
|
plc-complemented ΔpfoAΔplc |
JIR4461 |
JIR4444(pJIR1642), CmR |
plc+ |
6.8 ± 0.7 |
< 1.0 |
[23] |
| double-complemented ΔpfoAΔplc | JIR4462 | JIR4444(pJIR1720), CmR | pfoA+plc+ | 8.0 ± 0.5 | 3.5 ± 0.1 | [23] |
apfoA: perfringolysin gene, plc : alpha toxin gene, bCmr: chloramphenicol resistant, cPlc : alpha toxin, PFO: perfringolysin O, dJIR325 is a rifampicin and nalidixic acid resistant derivative of strain 13, a gas gangrene strain.