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PDF protein expression is elevated in colon tumor tissues. Western blotting was done to determine the expression of PDF in colon cancer tissue samples (T) relative to normal colon tissue (N) from two patients. Elevated PDF levels were found in the colon tumor samples for each patient. A β-actin antibody was used to confirm equal protein loading of the tissue samples for each patient. Two replicate experiments were performed and this image shows one representative experiment.
