Table 5. Novel miRNAs identified from castor bean.
| miRNA | Sequences (5′–3′) | Length(nt) | Reads | RNA* | No of loci | ||||
| leaf | root | seed1 | seed2 | endosperm | |||||
| Rco-miR001a | TTGGAGGATAGTTTCAGGCCGG | 22 | 0 | 127 | 0 | 19 | 0 | no | 1 |
| Rco-miR002a | GTGGACGTGCCGGAGTGGTTA | 21 | 1565 | 3188 | 0 | 1221 | 1656 | no | 2 |
| Rco-miR003b | TCTGATAGCAAAAGATAGAAC | 21 | 814 | 0 | 0 | 0 | 0 | no | 1 |
| Rco-miR004a | CAACGGATAGGTATACAGTTTT | 22 | 302 | 0 | 412 | 100 | 0 | no | 1 |
| Rco-miR005a | TCTGAAATTGCAGAGCCTAAA | 21 | 225 | 372 | 124 | 205 | 343 | no | 1 |
| Rco-miR006a | TCTTTGTAGTTTTGATCCGGAG | 22 | 1312 | 2054 | 1735 | 1394 | 1314 | no | 1 |
| Rco-miR007a | AGAGAAGGATGGTAGAGATGGTT | 23 | 10 | 0 | 27 | 0 | 276 | no | 2 |
| Rco-miR008a | TATCTTTGTAGTTTTGATCCGG | 22 | 322 | 705 | 547 | 0 | 0 | no | 1 |
| Rco-miR009a | TGAAGATGAAGAGCTATGTTTGA | 23 | 867 | 14 | 10 | 131 | 0 | no | 1 |
| Rco-miR010a | TGAGGAAGAGGATGACTTTGGA | 22 | 0 | 110 | 0 | 59 | 22 | no | 1 |
| Rco-miR011a | TCTCTAATTCGCTTGGTGCAG | 21 | 193 | 178 | 43 | 71 | 58 | yes | 1 |
| Rco-miR012a | CAATTGGATCGTTATTTGCTA | 21 | 113 | 137 | 87 | 167 | 132 | no | 1 |
| Rco-miR013a | AGGTGCAGGTGTGAGTGCAGG | 21 | 17 | 123 | 96 | 0 | 18 | yes | 1 |
| Rco-miR014a | TAATCTTGCTAACGGACTAAA | 21 | 29 | 163 | 0 | 0 | 55 | yes | 1 |
| Rco-miR015a | GCCGCTATGGTGAAATCGGT | 20 | 407 | 0 | 0 | 0 | 0 | yes | 1 |
| Rco-miR016a | AAGCCTGCGAGAGAGAGTTGG | 21 | 0 | 0 | 0 | 371 | 346 | yes | 1 |
| Rco-miR017a | AGGCCGATGACGATTAGAGGACG | 23 | 0 | 147 | 0 | 0 | 0 | yes | 2 |
| Rco-miR018b | TTCAAAAGGAGAACAAGGATAA | 22 | 457 | 0 | 0 | 0 | 0 | no | 1 |
| Rco-miR019a | ACATCCTTGAAGCTAACTCTA | 21 | 45 | 19 | 465 | 386 | 573 | yes | 1 |
| Rco-miR020a | AGGCAGTCATCTCTTGGCTAC | 21 | 0 | 0 | 0 | 0 | 163 | yes | 1 |
| Rco-miR021a | CGAGTCATCTGACAGAAGTAG | 21 | 0 | 443 | 0 | 0 | 0 | yes | 1 |
| Rco-miR022b | AGTGGGCGGAAAGGGGGGGTA | 21 | 189 | 0 | 0 | 0 | 0 | no | 1 |
| Rco-miR023a | TTTTATCACCGTCAGATTCTA | 21 | 127 | 333 | 77 | 221 | 185 | no | 1 |
| Rco-miR024a | TTTTGCCTACACCACCCATTCC | 22 | 0 | 637 | 621 | 0 | 0 | no | 4 |
| Rco-miR025a | AATAGTGATTGTGATATTGGCC | 22 | 323 | 0 | 10 | 10 | 0 | yes | 1 |
| Rco-miR026a | ATTTTAGGAAGGGAATGAACA | 21 | 249 | 768 | 368 | 653 | 431 | yes | 1 |
| Rco-miR027b | TTATTTTGATTTTGGACGTTTC | 22 | 180 | 0 | 0 | 0 | 0 | no | 5 |
| Rco-miR028a | TCTTATAGCAATCAGGGGACTTG | 23 | 0 | 16651 | 0 | 0 | 0 | yes | 1 |
| Rco-miR029a | TATGGGGGGATCGGGCAATAT | 21 | 3079 | 8498 | 6191 | 4222 | 2431 | yes | 1 |
| Rco-miR030a | GTCTGGGTGGTGTAGTCGGTT | 21 | 3842 | 3735 | 5213 | 5004 | 5369 | no | 1 |
| Rco-miR031a | TGTCGCTGGAGAGATGGCGCCA | 22 | 132 | 114 | 64 | 0 | 0 | no | 1 |
| Rco-miR032a | GAGGTCCTGTAGGGAGAGTGG | 21 | 14 | 33 | 11443 | 0 | 29 | yes | 1 |
| Rco-miR033a | TCCGGAGAGATTTGTGGACGA | 21 | 237 | 0 | 418 | 0 | 285 | no | 1 |
| Rco-miR034a | TCAGGTGGAGAATCAAACAGA | 21 | 171 | 0 | 167 | 600 | 419 | no | 1 |
| Rco-miR035a | TCCGGAGAGATTTGTGGACGAT | 22 | 0 | 0 | 418 | 0 | 285 | no | 1 |
| Rco-miR036a | CATGGACCAGAAGGCATATAC | 21 | 103 | 82 | 0 | 84 | 66 | no | 1 |
| Rco-miR037a | CTGAGACTTGAGGGATAGGTGTT | 23 | 0 | 579 | 111 | 0 | 0 | no | 5 |
| Rco-miR038a | TGACGTGGCATGAACTTCGGCA | 22 | 923 | 641 | 376 | 1013 | 1707 | no | 1 |
| Rco-miR039a | TAGAGCCAAGAATGACTTGCCGG | 23 | 0 | 0 | 0 | 204 | 411 | yes | 1 |
| Rco-miR040a | ACTCTCTCTGAAGGCTTCAAA | 21 | 3199 | 1179 | 935 | 4583 | 4525 | no | 1 |
| Rco-miR041a | TCCGGAGAGATTTGTGGACGAT | 22 | 418 | 0 | 515 | 0 | 285 | yes | 1 |
| Rco-miR042a | TCTGTCGCAGGAAAGATGGTAC | 22 | 0 | 3225 | 76 | 0 | 863 | yes | 1 |
| Rco-miR043a | TTTGCATGACCTGGGAGACGT | 21 | 81 | 9617 | 243 | 28454 | 25073 | no | 1 |
| Rco-miR044a | TGGAAATTTCTGGGTTGGAGG | 21 | 0 | 2789 | 2941 | 896 | 314 | no | 1 |
| Rco-miR045b | ATCAAATAAGGAAGAATCGAG | 21 | 0 | 0 | 0 | 121 | 0 | no | 1 |
| Rco-miR046b | TCGAAAGAGATATCAAGGACTG | 22 | 0 | 0 | 0 | 1789 | 0 | no | 1 |
| Rco-miR047a | GGAGGCCTTTGAGCAGAGTGGA | 22 | 0 | 0 | 118 | 40 | 0 | yes | 1 |
| Rco-miR048b | TTGGCATCAGAGGAGTCAAGC | 21 | 105 | 0 | 0 | 0 | 0 | no | 1 |
| Rco-miR049a | TAGGCAAAGCATCAGGATTCAT | 22 | 2121 | 434 | 0 | 0 | 0 | no | 2 |
| Rco-miR050a | TGTTTTTTGATCAGGACCATAA | 22 | 174 | 167 | 201 | 68 | 139 | no | 1 |
| Rco-miR051a | CTGTCGCAGGAGCGGTGGCACC | 22 | 687 | 523 | 23 | 0 | 0 | yes | 1 |
| Rco-miR052a | GGTATTGGACGGGTTGGCAAGA | 22 | 9127 | 19777 | 4389 | 8140 | 1429 | yes | 1 |
| Rco-miR053a | TCGAACCCAACTAGAAGATCTC | 22 | 0 | 0 | 1225 | 2281 | 1379 | no | 4 |
| Rco-miR054a | TATGGGAGGCATGGTCAGAAA | 21 | 290 | 5820 | 886 | 867 | 417 | no | 1 |
| Rco-miR055a | TGGACAAGTAGAGGTTACTAAT | 22 | 0 | 214 | 244 | 422 | 472 | no | 1 |
| Rco-miR056b | TCTGGATGAAGGCTGGAGTGAT | 22 | 0 | 0 | 549 | 0 | 0 | no | 1 |
| Rco-miR057a | GCCGCTATGGTGAAATCGGT | 20 | 407 | 17 | 0 | 15 | 0 | no | 1 |
| Rco-miR058b | TGAGGTTGGGTTGGACGACATA | 22 | 0 | 1470 | 0 | 0 | 0 | no | 1 |
| Rco-miR059a | CAGCAAGGATTAAGGGACATTT | 22 | 296 | 0 | 556 | 0 | 0 | no | 1 |
| Rco-miR060b | TCTGAAGCTGTGAATGGGAAT | 21 | 0 | 0 | 0 | 277 | 0 | no | 2 |
| Rco-miR061a | GAACGGCATTTGTAGCCCAGGAG | 23 | 101 | 35 | 17 | 10 | 0 | yes | 1 |
| Rco-miR062a | TCTGAATCAGGCTCTATATTAG | 22 | 0 | 53 | 0 | 159 | 0 | yes | 1 |
| Rco-miR063b | TTGAACAGTAGGAAGAGGGTTT | 22 | 0 | 0 | 0 | 328 | 0 | no | 1 |
| Rco-miR064a | TCTTTATATAGAGGTCTCGGAG | 22 | 2595 | 1375 | 1103 | 1600 | 1864 | no | 1 |
| Rco-miR065a | TTTTGTGCCAAGAACGTTGTTT | 22 | 237 | 121 | 48 | 0 | 198 | no | 5 |
| Rco-miR066a | TGGATAAGTTTCAGGAGATCTC | 22 | 667 | 833 | 795 | 822 | 0 | yes | 1 |
| Rco-miR067b | TGGGCTTTGAAGAAGAAGGTA | 21 | 0 | 0 | 110 | 0 | 0 | no | 1 |
| Rco-miR068a | TCATCAGATGAAGAGCATGACC | 22 | 1064 | 0 | 933 | 0 | 0 | no | 1 |
| Rco-miR069b | TGGGCTAGAGCATTAGAAGTTT | 22 | 0 | 0 | 129 | 0 | 0 | no | 1 |
| Rco-miR070a | TCTGGGAGTAGATTGAAGTGAA | 22 | 1182 | 0 | 0 | 1475 | 0 | no | 1 |
| Rco-miR071b | ATTGAGTTGGTAGAAGGTGCAA | 22 | 0 | 0 | 140 | 0 | 0 | no | 1 |
| Rco-miR072a | TTAGGAAAGCAGCTTGACACGTG | 23 | 0 | 0 | 36 | 189 | 0 | yes | 1 |
Note: a: these candidates meet Meyers et al.’s criteria; b: these candidates do not meet Meyers et al.’s criteria.