Figure 5.

Lowering incubation temperature from 37°C to 25°C does not affect leakiness in LacI^WF. GFP expression levels were measured on samples from cultures incubated without added IPTG at either 37°C (filled circles) or 25°C (filled squares) for both WT (dark blue/blue) and LacI^WF (dark red/red). For the mutant, expression levels in the absence of IPTG remain consistently 5-10 times lower than WT during the overnight incubation and are only marginally affected by temperature.