Table 1. Signaling pathways.
| WT_Mock | ERAP1-KO_Mock | WT_Ad5-HIV-gag | ERAP1-KO_Ad5-HIV-gag | |
| DAI | 1.0±0.1 | 1.6±0.7 | 87.2±15.6a | 106.4±36.6a |
| FynT | 1.0±0.1 | 1.0±0.3 | 0.5±0.1 | 0.7±0.3 |
| IRF3 | 1.0±0.3 | 1.0±0.3 | 1.0±0.3 | 1.4±0.5 |
| IRF7 | 1.0±0.2 | 1.1±0.1 | 15.1±2.5a | 23.1±7.3ab |
| IRF8 | 1.0±0.05 | 1.0±0.6 | 3.2±0.9a | 4.8±2.2a |
| Jak1 | 1.0±0.1 | 0.8±0.1 | 1.2±0.2 | 1.2±0.7 |
| MyD88 | 1.0±0.2 | 0.9±0.1 | 6.3±0.3a | 8.5±2.7ab |
| NFkB | 1.0±0.1 | 0.9±0.1 | 1.8±0.7 | 2.3±0.7a |
| NOD1 | 1.0±0.2 | 0.9±0.1 | 2.7±0.5a | 3.1±1.0a |
| NOD2 | 1.0±0.03 | 1.3±0.7 | 3.8±1.0a | 5.6±2.2ab |
| RIG1 | 1.0±0.2 | 1.5±0.3 | 13.2±4.1a | 17.3±5.0a |
| SLAM | 1.1±0.5 | 1.7±0.8 | 1.8±0.4 | 1.5±0.2 |
| SOCS1 | 1.2±0.8 | 1.1±0.7 | 209.6±72.9a | 217.7±95.1a |
| SOCS3 | 1.0±0.2 | 0.4±0.1 | 4.9±1.0a | 4.4±1.4a |
| STAT1 | 1.0±0.1 | 1.0±0.2 | 17.1±4.1a | 22.0±8.3a |
| TBK1 | 1.0±0.1 | 0.9±0.3 | 5.2±0.8a | 5.9±2.0a |
| TLR2 | 1.0±0.1 | 1.5±0.4 | 128.1±50.0a | 163.5±63.7ab |
| TLR3 | 1.0±0.2 | 0.8±0.4 | 27.8±12.4a | 37.8±17.9ab |
| TLR4 | 1.0±0.2 | 1.2±0.3 | 1.4±0.3 | 1.8±0.6 |
| TLR6 | 1.0±0.2 | 1.2±0.4 | 5.2±1.8a | 10.6±3.7ab |
| TLR9 | 1.0±0.4 | 1.8±0.8 | 8.2±2.7a | 6.3±2.5a |
| TRAF6 | 1.0±0.2 | 0.8±0.2 | 1.0±0.1 | 1.0±0.6 |
| TRIF | 1.0±0.3 | 1.0±0.3 | 1.7±0.4a | 2.0±0.8a |
Ad5-HIV-gag induced gene expression in livers of C57BL/6 mice (fold over WT_Mock, 6 hpi). The numbers represent Mean ± SD. Statistical analysis was completed using One Way ANOVA with a Student-Newman-Keuls post-hoc test, p<0.05 was deemed a statistically significant difference. n = 4 for all Mock injected groups, n = 6 for all Ad5-HIV-gag injected groups.
a
Significant differences as compared to WT_Mock;
b
significant differences in transcriptional activation in ERAP1-KO_Ad5-HIV-gag group as compared to WT_Ad5-HIV-gag group (also indicated by boldface font).