Figure 2. JEV replication and effects on permeability barrier in Caco-2 cells.

(A) Cells grown on trans-well inserts were infected with JEV with an MOI of 5 pfu/cell and viral titers in the apical and basolateral media was measured by plaque assay at the indicated times post-infection. (B) TER was measured from cells grown on trans-wells and infected with JEV as above. (C) JEV infected cells were incubated with soluble fluorescein and the amount of fluorescein passing from apical to basolateral side was measured as described in materials and methods. The figures are representative of three or more experiments performed with three or more replicates. Error bars indicate mean ± s.d. *** p<0.0001 as determined by two-tailed t-test.