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View full-text article in PMC

. 2013 Jul 24;8(7):e69632. doi: 10.1371/journal.pone.0069632

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© 2013 Segarra et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Tg338 127S infected brain homogenate dilutions (10⁻⁵ and 10⁻⁶) were PMCA amplified (one round) using 10% tg338 NBH (Undil.), 10% tg338NBH diluted 1/6 in 10% tg-PrP^0/0NBH and 10% tg338NBH diluted 1/8 in 10% tg-PrP^0/0NBH as PMCA substrates. The detection was performed on PK-digested amplified products using western blot analysis with 6D11 as the primary antibody. Undil.: 10% tg338 NBH without dilution in 10% tg-PrP^0/0NBH. NC: negative control, NBH only.