Figure 3. Capture optimization of ovine brain PrP^TSE.

3A: Batches of nanobeads (1% suspension) coated with 10, 20, and 30 µg of plasminogen/mg of beads were used to test prion capture efficacy using 30, 60 and 90 µl of plasminogen-coupled beads per 500 µl of human plasma spiked with a 10⁻³ dilution of 127S IBH. After one round of PMCA, detection was performed on PK-digested and amplified products using western blot analysis with 6D11 as the primary antibody. Lane 1: NBH: normal brain homogenate without PK digestion Lane 2: F10⁻² IBH dilution without PMCA (Frozen) Lane 6: negative control: plasma only 3B: Tg338 127S IBH dilution (10⁻⁴) in plasma was tested using different volumes of coated beads at 10 µg of plasminogen/mg of beads (1% suspension) for the prion capture. After one round of PMCA, detection was performed on PK-digested and amplified product using western blot analysis with 6D11 as the primary antibody. Lane 1: NBH: normal brain homogenate without PK digestion Lane 5: negative control: plasma only.