Figure 4. Efficacy of PrP^TSE capture.

Ten microliters of 10% 127S (lanes 1 and 2) and vCJD IBH (lanes 3 and 4) were diluted in 500 µl of plasma and incubated 2 h with plasminogen-coated beads. PrP^TSE bound to the beads were PK-digested and denatured in sample buffer for western blot analysis with 6D11 and 3F4 anti-PrP MAbs. Percentage yield was quantified with Genetools software after acquisition of the chemioluminescent western blot signals with the Genegnome digital imager (Syngene, US) Lanes 1 and 2∶127S IBH capture Lanes 3 and 4: vCJD IBH capture.