Figure 6. Detection of PrP^TSE from SWBC of scrapie-infected sheep.

Four infected and healthy SWBC (sheep white blood cells) samples underwent the capture step with 10 µl of coated beads at 10 µg of plasminogen/mg of beads. After three rounds of PMCA, PrP^res detection was performed after PK digestion of the amplified products, using western blot analysis with 6D11 as the primary antibody.