Figure 7. Detection of PrP^TSE from plasma and buffy coat of scrapie-infected sheep at the preclinical stage 7A:

500 µl of PG 127 infected and healthy plasma samples underwent the capture step in duplicate with 10 µl of coated beads at 10 µg of plasminogen/mg of beads, before PMCA amplification using two rounds of 80 cycles. Detection was performed after PK digestion of the amplified products using western blot analysis with 6D11 as the primary antibody. Pre-Cl-Plasma: plasma sample from PG127 infected sheep at the pre-clinical stage (120 days post oral challenge) Neg Plasma: plasma sample from healthy sheep 7B: 50 and 25 µl of PG 127 infected (120 days post oral challenge) and healthy BC (buffy coat) samples underwent the capture step in duplicate with 10 µl of coated beads at 10 µg of plasminogen/mg of beads, before PMCA amplification using one round (i.e. 80 cycles). Detection was performed after PK digestion of the amplified products, using western blot analysis with 6D11 as the primary antibody. Lane PC: 10⁻⁷ IBH dilution amplified by PMCA Lane NC: Buffy coat from healthy sheep.