Table 1. Evaluation of the PrPTSE detection assay on human blinded panel (NIBSC).
Biological materials | Dilutions | Positive |
vCJD brain spiked in plasma | 10−2 | 4/4 |
10−3 | 4/4 | |
10−4 | 4/4 | |
10−5 | 3/8 | |
10−6 | 1/4 | |
10−7 | 0/4 | |
10−8 | 0/8 | |
vCJD spleen spiked in plasma | 10−1 | 4/4 |
10−2 | 0/4 | |
10−3 | 0/8 | |
10−4 | 0/4 | |
10−5 | 0/4 | |
Control brain spiked in plasma | 10−2 | 0/4 |
10−3 | 0/4 | |
10−4 | 0/4 | |
Control spleen spiked in plasma | 10−1 | 0/4 |
10−2 | 0/4 | |
Control Plasma | – | 0/16 |
All the controls were collected from individuals showing no sign of vCJD (brain, spleen and plasma).