Figure 7. Model of the fate of linear DNA in S. cerevisiae and C. glabrata.

In S. cerevisiae, we were unable to observe re-circularization via NHEJ; however, it is likely that there is still a low level of NHEJ activity. Consequently, there is little change in the frequency of HR in both S. cerevisiae wild-type and dnl4Δ. In contrast, in C. glabrata, we observe insertion and vector ligation via NHEJ. Deletion of DNL4 results in an increase in HR, indicating that NHEJ is more prevalent in C. glabrata.