Figure 3. Physical exercise restores the expression of DNAJB3.
(A) Quantitative analysis of DNAJB3 mRNA levels in the adipose tissue from obese before exercise (n = 10) and after 3 months of exercise (n = 10) using real-time PCR. (B and C) Immunohistochemical staining using subcutaneous adipose biopsies from obese subjects before exercise (n = 11) and after 3 months of exercise (n = 7) using DNAJB3 (B) and Phopsho-JNK (C) antibodies. Arrows indicate the positive staining. Aperio software was used for quantification and the values are illustrated at the bottom as fold changes after exercise. Student's t-test for two group analysis was done to compare the expression of DNAJB3 (B) and JNK (C) in obese before and after exercise. *: P<0.05.
