Table 1. Relative expression analysis of genes associated with NER.

Gene Symbol	Definition	Fold change	p value
TP53	Tumor protein p53	1.83	0.017*
CDKN1A	Cyclin-dependent kinase inhibitor 1A	2.3	0.003*
RPA32	Replication Protein A	0.85	0.31
H2AX	H2A histone family, member X	1.21	0.17
PCNA	Proliferating Cell Nuclear Antigen	0.34	0.006*
POLH	Polymerase (DNA directed), eta	0.45	0.004*
DDB1	Damage-specific DNA Binding Protein 1	0.091	0.0001*
DDB2	Damage-specific DNA Binding Protein 2	0.62	0.0527
ERCC8	Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (CSA)	0.73	0.061
ERCC6	Excision Repair Cross-complementing rodent repair deficiency, Complementation group 6 (CSB)	0.42	0.015*
XPA	Xeroderma Pigmentosum, complementation group A	0.82	0.077
ERCC5	Excision Repair Cross-complementing rodent repair deficiency, Complementation group 5 (XPG)	0.83	0.098

Expression analysis of NER, cell cycle regulation and DNA damage detection factors in LB1 silenced and control cells. mRNA from Sc and shLB1 U-2 OS cells was prepared at 3 days after silencing and analyzed by qRT-PCR using GAPDH as a reference gene. The change in expression of a specific gene was considered significant if the “fold change” was higher than 1.7 or lower than 0.6.