Figure 4. Multiplex cytokine analysis of cell culture supernatants from fetal and adult fibroblasts and keratinocytes mono-cultures or fibroblasts and keratinocytes co-cultures.

Supernatants from fibroblast and keratinocyte mono-cultures or both cell types in co-culture (ratio 1∶1) were collected to detect soluble HGF, GM-CSF, IL-1α, VEGF-A and IL-8 by multiplex immunoassay (see materials and methods). For adult skin cells, values are the means of three samples (see table 1). Fib., fibroblasts mono-culture; Ker., keratinocytes mono-culture; Fib./Ker. (1∶1), fibroblasts and keratinocytes co-culture at the ratio 1∶1.