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. 2013 Jul 24;8(7):e69916. doi: 10.1371/journal.pone.0069916

Figure 12. Bioactivity of purified proteins on MDCK cells (AO/EtBr).

Figure 12

Effect of purified proteins MP1 and MP2 from human renal stone matrix on MDCK cells by AO/EtBr staining analysis of the induction of apoptosis in MDCK, visualized under fluorescence microscopy at magnification 200X. Viable cells are shown in green, apoptotic cells in fluorescent green and necrotic cells in orange. Cells were harvested at the indicated times and then stained with acridine orange (AO)/ethidium bromide (EtBr). Symbols and labels are used in the same way as in the figure 12.