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. 2013 Jul 24;8(7):e69916. doi: 10.1371/journal.pone.0069916

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© 2013 Aggarwal et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Effect of purified proteins NP1, NP2 and OP1 from human renal stone matrix on MDCK cells by AO/EtBr staining analysis of the induction of apoptosis in MDCK, visualized under fluorescence microscopy at magnification 200X. Viable cells are shown in green, apoptotic cells in fluorescent green and necrotic cells in orange. Cells were harvested at the indicated times and then stained with acridine orange (AO)/ethidium bromide (EtBr). Symbols and labels are used in the same way as in the figure 13.