Table 3. Reagent usage comparing conventional benchtop and on-chip library preparation protocols.

		Volume used per sample
Protocol step	Enzyme mix used	Manual protocol	AMCC chip protocol*
End repair	NEBNext End Repair Module	5 µL	0.31
dA Tailing	NEBNext dA-tailing module	3 µL	0.31
Adapter ligation	NEBNext Quick Ligation module	5 µL	0.31

* Reagent usage is based on loading 5 µL enzyme, which is distributed on-chip to all 16 reaction modules