Skip to main content
. 2013 Jul 24;8(7):e68838. doi: 10.1371/journal.pone.0068838

Figure 3. CRNN inhibits tumor formation in nude mice.

Figure 3

(A) Representatives of tumors formed in nude mice induced by vector-transfected KYSE30 cells (left) and CRNN-transfected cells (right), respectively. Representatives of tumors derived from tested animals were shown in the upper panel. Tumor volumes were summarized in the lower panel. *, P<0.05; **, P<0.01. (B) Similar results were obtained in CRNN-transfected KYSE180 cells compared with vector controls. *, P<0.05; **, P<0.01. (C) IHC staining was performed to confirm the expression of CRNN in the tumors induced by CRNN-transfected cells but not in the tumors induced by vector-transfected cells. Original magnification, 200×magnification. (D) Representative and summary of DNA content detected by flow cytometry. The percentage of cells in S phase was significantly decreased in CRNN-transfected cells compared with control cells. *, P<0.05; **, P<0.01. (E) Western blot analysis shows that CRNN could upregulate P21WAF1/CIP1 and Rb expression significantly. P53 also increased slightly in CRNN-transfected cells. Tubulin was used as a loading control. p21 mRNA level was determined by qRT-PCR. *, P<0.05; **, P<0.01.